Page 1. Start Analysis
If the titration curve is pasted into the active grid, enter experimental parameters and click Calculate Q. The titration curve will be converted to the binding isotherm Q.
After data are entered, click Calculate pK distribution to initiate the analysis.
Page 2. Solution
The initial solution applies an initial guess for Lambda parameter based on the L-curve and Fitting error analysis. The user can interactively select other Lambda values to see the effect on the pK distribution and fit.
Save results as… >> Save results files to be used for overlays.
Export CSV >> Generate CSV file.
Page 3. Overlays & Peak Integration
Page 4. Tab Results
View numerical results.
Select columns >> Copy to clipboard.